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A recombinant vaccinia virus expressing canine interferon (IFN)-β was constructed (vv/cIFN-β). In rabbit kidney (RK13) and canine A72 cells infected with vv/cIFN-β, the recombinant canine IFN-β was detected in both cell extracts and supernatants, and the IFN activities of the culture supernatants were also detected. Inhibition of N-linked glycosylation by tunicamycin treatment indicated that the recombinant...
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