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Specific binding of 125 I-labelled GLP-1(7-36)amide to rat lung membranes was dependent upon time and temperature and was proportional to membrane protein concentration. Binding was inhibited in a concentration-dependent manner by unlabelled GLP-l(7-36)amide consistent with the presence of a single class of binding sites with a dissociation constant (K d ) of 1.67 +/- 0.29...
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