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The spatial organization and the antigenic structure of the bacteriorhodopsin molecule in the purple membrane were studied by immunochemical techniques. Five monoclonal antibodies directed against exposed parts of the protein molecule in the membrane were prepared and characterized. Antigenic determinants were localized in the bacteriorhodopsin polypeptide chain by analysis of the interaction between...
A monoclonal antibody technique for determining the surface morphology of the purple membrane (PM) has been developed. Two types of antibodies for distinguishing the extracellular surface and the cytoplasmic surface of PM were produced by immunizing the corresponding synthetic peptides to mice. The Langmuir-Blodgett (LB) method was employed to form a single-layer two-dimensional array of PM fragments...
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