Plants continuously are challenging biotic and abiotic stresses to survive. Reactive oxygen species (ROS) as hydrogen peroxide are a key signal to transduce environmental stimuli in plants response to stresses. Transgenic tobacco to CchGLP gene from C. chinense BG-3821, produce constitutive levels of hydrogen peroxide which correlates with tolerance to geminiviruses infections. In the present work, it was demonstrated that these transgenic tobaccos are also tolerant to drought stress. Moreover proteomic and metabolomic studies showed that both Omic profiles in tobacco tolerant to both biotic and abiotic stresses, displayed components and impact on biochemical pathways that likely explain the observed phenotype of tolerance in regards to no-tolerant lines and wild type tobaccos. Our results suggested that hydrogen peroxide levels within these studied plants, correlates with induction of proteins and metabolites related to plant defense to stress, opening the possibility that controlling somehow (i.e. applying elicitors in a controlled way) hydrogen peroxide or ROS levels within plants, might help to protect plants in an scenario of global warming for future agriculture.