Spectral line interference may occur in atomic absorption spectroscopy, when there is a significant overlap of the emission line profile with the absorption line profiles of any interfering species in the flame. The presence of the spectral interference can cause errors in the measurements of the net absorbance, and in the background correction.Both theoretical calculations and experimental observations reported in this paper provide a remark of this interference effect. It can be concluded from the experimental results, that the spectral interference is located close to the resonance wavelength of the analyte.Two different examples representing various types and degrees of spectral interference are discussed.