The kinetics of the monolayer formation of zinc oxide nanoparticles on water subphase was studied at different sample concentrations and barrier speeds. The recorded isotherms indicate that stearic acid can act as a suitable microenvironment for the deposition of zinc oxide when the spreading solution contains ~50% sample. The AFM image of the double layer shows that the film is nearly homogeneous with average thickness ~15nm. The sensing ability of the nano-films was studied by cyclic voltammetry using ITO substrates, coated with zinc oxide nanoparticles as working electrode. It gave different reduction peaks for different pH electrolytes.