This study was performed to determine the existence of hysteresis during ultrasonic velocity measurements on methanol, ethanol, distilled water, processed water and glycerol under thermal cycle (5–50°C). The ultrasonic velocity of the liquids were measured with 1.0MHz transducers using a through transmission ultrasonic system. Statistical analyses results showed that there were ultrasonic velocity hystereses on the liquid samples under thermal cycle. The study of liquids undergoing thermal cycle is useful for determining the temperature effects on ultrasonic velocity and will help developing precise control applications in food industry by reducing the uncertainties in ultrasonic velocity measurements.