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The following structure of the O-specific polysaccharide of Proteus mirabilis O5 was established by 1 H and 13 C NMR spectroscopy at 500 MHz, including two-dimensional COSY, TOCSY, NOESY, and H-detected 1 H, 13 C heteronuclear multiple-quantum coherence (HMQC) experiments: where O-acetylation of α-d-GlcNAc at both positions is nonstoichiometric.