The interaction of EG, DEG and TEG with biologically active D-Panthenol, as the function of temperature has been investigated by the combination of acoustic and volumetric methods. Speeds of sound and densities of EG, DEG and TEG in (0.05, 0.10 and 0.15) mol·kg−1 in aqueous solutions of D-Panthenol have been measured at T = (293.15, 298.15, 303.15 and 308.15) K. From the density data, the apparent molar volume (Vϕ0) and standard partial molar volume of transfer ΔVϕ0 for EG, DEG and TEG in aqueous D-Panthenol solutions have been determined. From the speed of sound data, the partial molar isentropic compression Kϕ,s and the partial molar isentropic compression of transfer ΔKϕ,s0, have been determined. The pair and triplet interaction coefficient have also been estimated.