Studies on the mechanisms of mineralization of connective tissues, have gained momentum in the recent past. In the present study, Biomimetic mineralization of modified fish scale collagen in vitro is reported. The fish scale collagen was crosslinked with 3-Aminopropyl triethoxysilane and the crosslinked collagen (FCSi) was characterized using conventional methods. The biomimetic mineralization capacity of FCSi was evaluated in SBF solution for 7days. Formation of hydroxyapatite crystals on the matrix in vitro has been confirmed by FTIR, XRD and SEM-EDAX techniques. The FCSi may be used as a scaffold in bone tissue engineering and as an osteoinductive material in experimental animal models before applying clinically.