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To elucidate the role of mitochondrial DNA (mtDNA) in determination of growth of hepatocellular carcinoma, we examined wild-type Hepa1-6 cells and their ρ 0 cells with depleted mtDNA in vitro and in vivo. Cultured ρ 0 cells grew more rapidly than did wild-type cells. Production of reactive oxygen species (ROS) was higher in wild-type cells than in ρ 0 cells. Hypoxia inhibited...
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