To determine optimum periods for 18 F-fluorodeoxyglucose (FDG) positron emission tomography (PET) examination in subjects with suspected acute myocarditis, we compared 18 F-FDG PET with endomyocardial biopsy (EMB) using the latest definition of 18 F-FDG PET for inflammatory left ventricular (LV) myocardium.Retrospective analysis of 29 subjects (18 male, 48±18years) who have symptoms or LV dysfunction underwent both 18 F-FDG PET (Advance NXi, GE-Healthcare) under fasting conditions and EMB from LV posterior wall within 3months.When we defined 18 F-FDG PET positive inflammatory LV posterior wall as ‘focal on diffuse’ pattern, sensitivity, specificity, and positive predictive values (PPV), and negative predictive values (NPV) of 18 F-FDG PET for detecting active inflammatory LV posterior wall compared with EMB were 46.2, 81.3, 66.7, and 65.0%, respectively. Receiver operating characteristic curve of periods (days) between onset of clinically suspected acute myocarditis and performance of 18 F-FDG PET for detecting inflammatory LV posterior wall demonstrated 17days as a best cut off values with area under the curve (0.497, P=0.982) with sensitivity=21.1% and specificity=100%. Sensitivity, specificity, PPV and NPV of 18 F-FDG PET for detecting inflammatory LV posterior wall on EMB were all 100% when 18 F-FDG PET was performed at 1–14days after onset of suspected acute myocarditis.In our definition, 18 F-FDG PET showed excellent agreement with EMB for detecting active inflammatory LV posterior wall in subjects with clinically suspected active acute myocarditis. If possible, 18 F-FDG PET should be performed within 14days after the onset to maintain high diagnostic accuracy compared with EMB.