Atrazine is a common agricultural herbicide that is readily transported into estuaries through surface water runoff. In this study, we determined the short-term (24-48 h) sublethal effects of atrazine on estuarine phytoplankton biomass and community composition. Phytoplankton group-specific responses to atrazine exposure (25 μgl - 1 ) were measured using natural water samples collected from two locations in Galveston Bay, Texas. Addition bioassays, coupled with HPLC pigment analysis, were used to quantify changes in the relative abundances of algal groups. For all algal groups except prasinophytes, the addition of atrazine in combination with nitrate was not significantly different from nitrate additions alone. These results suggest no significant negative effect of atrazine on phytoplankton under the specified environmental conditions for the bioassays. Although low concentrations of atrazine may have minimal impacts on phytoplankton, herbicide loadings need to be further characterized before generalizations can be applied to estuarine and coastal ecosystems.