This study deals with preparing powder metallurgy Ti2AlNb-based alloy via spark plasma sintering (SPS) technique. A pre-alloyed Ti2AlNb-based alloy powder was produced by a plasma rotating electrode process (PREP), and then consolidated using SPS at temperatures between 900°C and 1050°C with a pressure of 50MPa. Most of the pre-alloyed powder particles are characterized by single B2-phase dendrites. When the SPS temperature was 1000°C or higher, the dendritic features and original boundaries of powder particles were removed, resulting in fully dense compacts. The as-sintered microstructure was modified by heat treatment to form fine acicular O-precipitates, leading to a superior yield strength of up to 1220MPa at room temperature (RT). On the other hand, the heat treated microstructure with coarsened O-precipitates, produced via controlled slow cooling within the B2+O phase field, exhibits a good RT elongation to fracture of 9.5%, but a relatively low yield strength of 840MPa.