Makarkinia kerneri sp. nov. is described from the Lower Cretaceous Crato Formation of Brazil, based on an incomplete but very well-preserved hind wing (Neuroptera: Kalligrammatidae). The previously presumed attribution of the genus is confirmed, based on the diagnostic characters of its wing venation (e.g., dense crossvenation; the anteriorly directed branches of MP) and the presence of a distinct eye-spot on the wing. Makarkinia is the only American representative and youngest known genus of Kalligrammatidae. With an estimated wing length of 100–160 mm, it has the largest wings amongst all fossil and extant Neuroptera.