The present study reports an environmental friendly and cost effective method for green synthesis of rutile TiO2 nanoparticles (NPs) from titanyl hydroxide using Euphorbia heteradena Jaub root extract as a reducing and capping agent. The hydroxyl functional group of phenolics in E. heteradena Jaub root extract act as reducing and capping/stabilizing agents. Also, the kinetics of the reaction was studied using UV–visible spectrophotometry which revealed surface plasmon resonance (SPR) around 360nm. The crystalline nature of TiO2 NPs was identified using XRD analysis which confirmed the formation of rutile phase. The possible mechanism leading to the formation of TiO2 NPs is suggested. The synthesized TiO2 NPs by this method is quite stable under inert atmosphere for two months.