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Oxygen free radicals are very reactive molecules which can react with every cellular component. They are normally produced in organisms being involved in various biologic reactions. However, too high levels of these partially-reduced O 2 species can give rise to functional and morphologic disturbances in cells. There is evidence to implicate oxygen free radicals as important pathologic...
The extracellular slime layer of Staphylococcus epidermidis ATCC 35983 contains: a) two non-anionic carbohydrate containing proteins degradable with papain of molecular masses 250 and 125 kDa; b) a polydisperse but homogeneously-charged acidic population with M r ranging from 120 000 to 35 000 (average M r 80 000) containing a polysaccharide coval smallpeptide; c) a papain degradable...
Wheat germ lysate was used as a model system for in vitro translation. We show that an increase of the exchange surface between the reaction mixture and the atmosphere enhanced the amount of incorporated cysteine, indicating that early arrest of protein synthesis previously observed in such a system was due to oxygen starvation in the reaction mixture. This hypothesis was confirmed since the amount...
The distribution of phosphatidylethanolamine (PE) and phosphatidylcholine (PC) among the outer and inner monolayers of the vacuolar membrane of Acer pseudoplatanus was investigated using isolated vacuoles, chemical labelling agents (trinitrobenzenesulfonate and fluorescamine), phospholipase A 2 from bee venom, phospholipase C and phospholipase D. Treatments were performed with intact or sonicated...
Previous attempts to study the binding of yeast ribosomal protein L1 with 5S rRNA in vitro have been impeded by the failure to form RNA-protein complexes with purified protein and RNA. To circumvent this difficulty, we have developed an in vitro system that allowed RNP formation. The system involved in vitro expression of the protein L1 from its cloned gene in the presence of exogenous yeast 5S...
Basic fibroblast growth factor (FGF2) is involved in both cell proliferation and differentiation processes. Heparin may interfere in the stability and biological activities of FGFs. However, it is difficult to obtain FGF preparation without traces of heparin since heparin affinity chromatographies are routinely used to prepare this growth factor. We have therefore devised a means of production...
Brush-border membrane vesicles (BBMV) were isolated from prawn hepatopancreas as we previously described (Muriana et al (1993) J Biochem 113, 625-629). The characterization of hepatopancreatic BBMV (hBBMV) by monitoring the activity of marker enzymes indicated a relatively pure apical membrane preparation reduced in basolateral contamination. Phospholipid composition of hBBMV was examined by the...
The superimposable dinucleotide fold domains of MetRS, GlnRS and TyrRS define structurally equivalent amino acids which have been used to constrain the sequence alignments of the 10 class I aminoacyl-tRNA synthetases (aaRS). The conservation of those residues which have been shown to be critical in some aaRS enables to predict their location and function in the other synthetases, particularly: i)...
A proposal to use a unified description of protein structures is presented, assuming that all peptide chain conformations (including β-structure) may be considered as helix-derived. In this understanding the β-conformation of the peptide chain may be obtained by extending or unwinding the helical form. A unified description could be based on a commonly defined internal axis (Z-axis) determined by...
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