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The purpose of this research was to study the effects of freezing as pre-treatment prior to pulsed electric field (PEF) on the quality of beef semitendinosus muscles. Fresh and frozen-thawed samples were treated using square -wave bipolar pulses at electric field strength 1.4kV/cm, pulse width 20μs, frequency 50Hz and total specific energy 250kJ/kg. PEF caused significant microstructural changes of...
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