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A rat jun-D genomic clone was isolated from a rat liver λEMBL3 library. Sequence analysis revealed a high sequence similarity with mouse jun-D, and relatively low similarity with human and chicken jun-D. In both the trans-activation and DNA-binding domains, however, the amino-acid sequences were well conserved among rat, mouse, human and chicken.
A cDNA encoding a zebrafish Sox protein (Sry-type high-mobility-group box) was isolated and sequenced. The sequence within the HMG box is close to those of the B subfamily comprising mouse Sox-1, Sox-2, Sox-3 and Sox-14. While much of the rest of the zebrafish protein is unique, there are blocks of amino acids showing considerable identity with regions of Sox-1, -2 and -3. These domains may represent...
The purpose of this study was to isolate the stage-specifically expressed Chironomus thummi thummi globin-encoding gene (Gb) 6 (ctt-6). The final product of this gene is hemoglobin (Hb) CTT-VI, a protein that is phylogenetically most closely related to Hb CTT-VIIB. In the absence of chromosomal rearrangement, genes of immediate common ancestry should be closely linked. This was shown for a genomic...
Two c-myc genes have been isolated from a tetraploid fish, the common carp (Cyprinus carpio). The nucleotide (nt) sequences of the two genes and their flanking regions (CAM1, 8675 bp and CAM2, 5784 bp) have been determined. Both genes contain two coding exons which are homologs of c-myc exons 2 and 3 in higher vertebrates. The nt sequence homologous to c-myc exon 1 of higher vertebrates has not...
The promoter regions of the genes encoding the rat and chum salmon growth hormones (GH) and rat prolactin (PRL) were combined with a reporter gene and introduced into GH- and/or PRL-producing cells from rat. The rat GH and PRL promoters (pGH and pPRL, respectively) were most active in cells producing GH and PRL, respectively. The activity of the salmon pGH was much less than that of the rat pGH...
A genomic clone containing the 5 -flanking sequence of the chicken GABA A receptor α1-subunit-encoding gene (GabRα1) was isolated and characterized. An intron was found to interrupt the 5 -untranslated region. The transcription start point (tsp) was determined by primer extension, RNase protection and the amplification of chick brain first-strand cDNA. DNA sequence analysis revealed a...
A cDNA encoding ribosomal (r) protein L18 of Arabidopsis thaliana (At) was isolated and characterized. The nucleotide sequence contains a 563-bp open reading frame that encodes a 20.9-kDa basic protein. Amino-acid comparison indicated that the predicted L18 r-protein of At has a high degree of homology with L18 of distantly related organisms such as yeast, Xenopus laevis and rat. Genomic DNA analysis...
We have characterized the immediate (465 bp) 5 -flanking region of the rat phenylalanine hydroxylase (PAH)-encoding gene. This sequence shows considerable similarity to the 5 -flanking region of the human PAH gene [Konecki et al., Biochemistry 31 (1992) 8363-8368]. Both sequences lack obvious TATA elements; however, putative regulatory sites, including a potential cyclic AMP-response element and...
A screening strategy designed to identify cloned cDNAs encoding abundant Macaca fascicularis epididymal transcripts has yielded a clone corresponding to a 1.03-kb transcript present at high levels in the epididymis. Following library rescreening, DNA sequence analysis of several near full-length clones predicts a novel, 151-amino-acid protein, epididymal secretory protein 14.6 (ESP14.6), which contains...
The K14 keratin is an intermediate filament produced in squamous epithelia. This tissue-specific expression is directed by the promoter (pK14) of the K14 gene which has been used extensively to direct the expression of transgenes to the skin. Human K14 was cloned and the upstream sequence is presented. In transient transfections, pK14 directs expression of a luciferase reporter in keratinocytes...
An examination of mRNA expression of the serotonin genes encoding both ψ5HT 1Dα and the related 5HT 1Dα , demonstrated that the pseudogene is transcribed, and has a tissue distribution similar to the 5HT 1Dα receptor-encoding gene. This ψ5HT 1Dα transcript is capable of being translated into a polypeptide of only 28 amino acids...
We report a striking amino-acid sequence similarity between a chicken extracellular matrix protein termed ES/130, involved in early cardiac morphogenesis [Rezaee et al., J. Biol. Chem. 268 (1993) 14404-14411], and a novel human protein termed CG-1, isolated from the glycoprotein fraction of peripheral blood leukocytes [Print et al., Gene 144 (1994) 221-228]. These two proteins may be homologues which...
Activating transcription factor 2 (ATF2) is a cellular sequence-specific DNA-binding protein that mediates transcriptional activation by the adenovirus (Ad) E1A protein. In injected Xenopus laevis oocytes, E1A-induced transactivation requires an ATF2 recognition sequence within the responding promoters, thereby suggesting that ATF2 is present in oocytes and perhaps has a developmentally important...
The Arabidopsis thaliana (At) 2S albumin gene 3 (At2S3) has been cloned in YEp13 as a 3.5-kb genomic fragment. To study its expression in Saccharomyces cerevisiae, the accumulation in saturated cultures reached about 0.032% of the yeast total protein, and the product was localized in vacuolar bodies within the cell. The 13-kDa protein was processed to 9- and 4-kDa proteins, as obtained in transgenic...
We have amplified and sequenced parts of the genomes of eleven laboratory strains of bovine viral diarrhea (BVD) virus originating from North America, New Zealand and Europe. The cumulative nucleotide (nt) sequence heterogeneity of the amplified fragments located in the analysed region of the gene encoding the nonstructural protein NS3 (P80) was 24% as compared to 47% for E2 (Gp53). The nt substitutions...
Two basic helix-loop-helix (bHLH) transcription factors, E47 and E12, are involved in cell-specific gene expression as part of dimeric complexes which interact with the cis-acting motif E-box. Although both generated from a single gene (E2A) by means of alternative splicing, the structural difference in these bHLH regions between the two suggests that the two bHLH proteins may differ in some of their...
An abundantly secreted 47-kDa glycoprotein, DS47, was purified from Drosophila melanogaster (Dm) Schneider line-2 cells, a line exhibiting macrophage-like properties. DS47 is also secreted from several Dm cell lines resembling S2 but not from lines that are morphologically distinct. A cDNA clone was isolated from an S2 cell cDNA library using oligodeoxyribonucleotide probes based on the DS47 amino...
The structure of Id was examined by Southern analysis in inbred mouse lines which included five subspecies of Mus musculus. No variation was detected within this species. The species Mus cookii shares the same form found in mice ofM. musculus derivation, indicative of a long evolutionary history and a common origin. However, five TaqI polymorphisms were found among several Mus species, suggesting...
A re-examination of the IS50 sequence revealed nine single-bp changes from the original published sequence. The changes account for four replacements in the deduced amino-acid sequences of the Tn5 transposase and inhibitor protein. Both IS50L and IS50R are 1534 bp in length, and the total length of Tn5, therefore, is 5820 bp.
The genome of the Australian bushfly Musca vetustissima contains a sequence that is highly similar to the transposase-encoding of the hobo, Ac and Tam3 (hAT) family of transposable elements.
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