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A cDNA encoding a novel mammalian member of the Clp/HSP104 family was isolated from a mouse macrophage-like cell line (J774.1) cDNA library by suppression of the growth defect of a Saccharomyces cerevisiae trkl trk2 double mutant. The full-length version of this cDNA, termed SKD3, encodes a putative 76-kDa protein of 677 amino acids (aa). The deduced aa sequence of the SKD3 polypeptide contains four...
The chaperonin containing t-complex polypeptide 1 (TCP-1), as one of its subunits, CCT, is a cytosolic heterooligomeric molecular chaperone assisting in the folding of proteins in eukaryotic cytosol. We have isolated a Tcp-1-related 119-bp cDNA fragment from a human cDNA library by polymerase chain reaction, and cloned full-length mouse cDNAs orthologous to the human cDNA by hybridization. The nucleotide...
Cell- and stage-specific transcription of the myelin basic protein (MBP)-encoding gene (Mbp) in brain is regulated by arrays of cis-acting regulatory elements positioned at the 5 -flanking region of the gene. The proximal element between nucleotides -14 to -50, termed MB1, has previously been shown to have an important role in the cell-type-specific transcription of Mbp in cells derived from the...
Using a rapid amplification of cDNA ends (RACE) cloning approach, we have isolated a cDNA clone encoding Rab19, a novel small GTPase of the Rab subfamily contained within partial sequences previously described [Chavrier et al., Gene 112 (1992) 261-264]. Northern blot analysis of the distribution of the rab19 mRNA in various adult mouse tissues and NIH 3T3 fibroblasts revealed that rab19 is expressed...
The third intron of the mitochondrial benzodiazepine receptor (MBR)-encoding gene was sequenced from hamster, mouse and human. The rodent species were found to include an Alu-like sequence, as was first discovered in the rat gene. Differences with the rat intron were evident by an insertion of an additional B1 element in the hamster and the introduction of a complete and two partial B2 sequences...
We have cloned and sequenced two new cDNAs that code for proteins carrying the related triple LIM domains (acronym of Lin-11, Isl-1, Mec-3) proteins. These LIM domains show good agreement to the LIM domain consensus sequence, but also exhibit some novel variations. The 1.36-and 2.8-kb cDNAs are probably splice variants of one gene and code for 42- and 50-kDa proteins, respectively. The larger transcript...
We have studied the integration of adenovirus type 12 (Ad12) DNA in transformed and hamster tumor cells over many years. Upon infection of hamster cells with Ad12, viral DNA has been found in association with hamster chromosomes, possibly in part integrated into the host genome. Ad12 DNA integration is not sequence specific. Transcriptionally active sites of the host genome show a preponderance...
Using the polymerase chain reaction with degenerate oligo primers we identified six new Antennapedia-like homeobox sequences expressed in a regenerating planarian. cDNA fragments containing the entire homeobox sequences for four of these genes were obtained. Two of them, Dutarh-3 and Dutarh-4, belong to the classical Antennapedia type and display extensive identity with Antennapedia- and Deformed-type...
The 13.2-kDa subunit of NADH:ubiquinone oxidoreductase has been shown to be an integral part of the bovine iron-sulfur (IP) part of the protein. This subunit has been shown to interact with at least two other protein subunits of the IP fragment. The amino acid (aa) sequence of this subunit, determined from an acid extract of rat heart was used to generate an oligodeoxyribonucleotide probe which...
Peripherin (Prph) is a type-III intermediate filament (IF) protein principally synthesized in peripheral nervous system neurons. We have previously shown that three regulatory elements, PER1, PER2 and PER3, in the first 98 bp of thePrph gene promoter, were sufficient to direct cell-type specific expression of a reporter gene [Desmarais et al., EMBO J. 11 (1992) 2971-2980]. Of these elements, PER1...
The newly identified Sox gene family (Sry-like HMG-box gene) is characterized by a conserved DNA sequence encoding a domain of approx. 80 amino acids (aa) which is responsible for sequence-specific DNA binding. The first member isolated, the mammalian Y-linked testis-determining gene, Sry, is necessary and sufficient for male development. We report here the identification of two new members of this...
We published a paper (Monroe and Huber, 1994) under the title The major form of the murine asialoglycoprotein receptor: cDNA sequence and expression in liver, testis and epididymis . This paper was submitted to Gene on December 28, 1993 and was accepted for publication on February 21, 1994. The essence of this paper was to document that the liver-specific asialoglycoprotein receptor is definitely...
We have isolated a cDNA corresponding to the hamster peroxisome proliferator-activated receptor haPPARγ, a member of the steroid nuclear hormone receptor superfamily of transcription factors. haPPARγ mRNA is highly expressed in adipose tissue, and is expressed in lung, heart, kidney, liver and spleen to a lower extent. Thus, haPPARγ may function in activating the transcription of target genes in a...
The 20-kDa ubiquitin-conjugating enzyme E2-20K is induced specifically during a late stage of erythroid differentiation. Here we report the sequence of a murine cDNA encoding E2-20K. Northern blot analysis identified polyadenylated transcripts of 3.5 and 6.5 kb which are present at comparable levels in many nonerythroid tissues.
A 5 truncated caprine (ca) -casein-encoding gene ( Cas) was fused to the 3 end of a 3 truncated ca βCas. The Cas form comprised the 0.8-kb 3 end of intron 2, the remaining part of the transcription unit containing codon -- 2 to stop 172, and 0.43 kb of the 3 flanking region. The βCas form comprised a 3-kb 5 flanking region and the 5 end of the transcription unit terminating 69...
We cloned the human cDNA corresponding to the cDNA (PEBP2αB-451) encoding the mouse polyoma virus enhancer-binding protein 2αB-451, representing a major splice variant from acute myeloid leukemia gene 1 (AML1). Genomic DNA clones of AML1 were also isolated and the exon/intron structure was determined. Furthermore, we determined and compared the genomic structures of three mammalian Runt domain-containing...
We compared (i) the enhancer/promoter (mCMV promoter) from the murine cytomegalovirus (CMV) major immediate early gene, (ii) the enhancer/promoter from the human CMV major immediate early gene, containing a short promoter (h1CMV) or a long stretch of 5 untranslated region (UTR) from the gene promoter (h2CMV) and (iii) the simian virus 40 (SV40) enhancer/early region promoter (SV2) for their ability...
The cDNA of the murine counterpart of the human TB2/DP1 (deleted in polyposis) gene, one of the six genes deleted in severe cases of familial adenomatous polyposis (FAP) disease, was isolated and analyzed. The murine transcript is 734-bp long and thereby considerably shorter than the 3100-bp human counterpart. This is due to a completely different 3' untranslated region in mouse which starts immediately...
Murine YRNAs, which are components of the conserved Ro ribonucleoprotein (RNP) complex, have been identified by enzymatic RNA sequencing. Mouse Y1 (mY1) and Y3 (mY3; originally named mY2) RNAs share 97 and 95% identity to the human Y1 and Y3 RNAs, respectively. TATA-like sequences, Proximal Sequence Elements, and octamer sequences, which are upstream promoter element motifs indicative of Class 3...
The major milk whey protein of ruminants is β-lactoglobulin. Ovine β-lactoglobulin-encoding gene expression is restricted to the sheep mammary gland. This report describes the expression profile of a truncated β-lactoglobulin transgene which, although not expressed in the mammary gland, is expressed in the kidney in the majority of lines generated. The high frequency of ectopic kidney expression...
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