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A full-length cDNA encoding murine tetranectin (TN) was isolated and cloned from a murine lung λZAPII cDNA library. The complete nucleotide sequence was determined revealing an open reading frame encoding 202 amino acids (aa) including a signal peptide of 21 aa. An overall aa identity of 79% exists between the deduced aa sequences of human and murine TN, revealing a high evolutionary conservation...
Candida albicans (Ca) FAS2, the gene encoding the α-subunit of fatty acid synthase (FAS), has been isolated and characterized. Saccharomyces cerevisiae (Sc) FAS2 was used as a probe to screen genomic libraries of Ca, strain 4918. Clones were obtained that contained all but the first 1 kb of the gene. The 5 end, as well as upstream sequences, were subsequently isolated by PCR. The Ca FAS2 gene...
We have cloned and analyzed the chicken c-sno (cellular ski nvel) gene. The promoter region and all of the intron/exon boundaries have been sequenced. The gene is approx. 12-kb long and contains six exons, the first of which is noncoding. The amino-acid sequences encoded in this first coding exon of c-sno and c-ski are highly related; however, the remainder of these two genes appears to be unrelated...
An Escherichia coli RFL47 DNA fragment containing the Eco47IR and Eco47II restriction-modification (R-M) system has been cloned and sequenced. A clone carrying this system has been selected by its ability to restrict phage λ in vivo. The sequence of 5360 bp was determined, and its analysis revealed three major open reading frames (ORF) corresponding to two restriction endonucleases (ENases) and...
In order to investigate the genetic basis for the observed polymorphism amongst meningococcal transferrin-binding proteins, Tbp2, the corresponding genes of different Neisseria meningitidis strains were cloned and sequenced. Comparison of the deduced amino acid (aa) sequences indicated that the Tbp2 were 76.6 to 81.2% homologous. Several stretches of aa have been found repeated both in the N- and...
The endogenous inhibitor of cAMP-dependent protein kinase (PKA) is down-regulated in the kidneys from vitamin-D-replete chicks as compared to vitamin-D-deficient chicks. Screening of a vitamin-D-deficient chick kidney library resulted in the isolation of a 450-bp cDNA clone encoding the 76-amino acid (aa) protein kinase inhibitor (PKI). The deduced aa sequence of avian PKI shares 80 and 41% identity...
Type-II restriction-modification (R-M) systems comprise two enzymes, a DNA methyltransferase (MTase) and a restriction endonuclease (ENase), each of which specifically interact with the same 4-8-bp sequence. All type-II MTases share several amino acid (aa) sequence motifs, which makes an evolutionary relatedness among these enzymes probable. The type-II ENases, in contrast, except for some homologous...
A murine cDNA sequence (termed pT49), encoding a protein related to the fibrinogen (Fib) β and γ chains, has been previously reported [Koyama et al., Proc. Natl. Acad. Sci. USA 84 (1987) 1609-1613]. We used a murine pT49 probe to screen a human small intestine cDNA library, and obtained a set of overlapping cDNA clones encoding a Fib-like protein that is 80% identical with the product of the murine...
A cDNA of the rat retinoblastoma gene (RB) was prepared from total RNA of rat liver using reverse transcription-polymerase chain reaction (RT-PCR). The 4432-nt sequence isolated contained 2700-nt translated and 1732-nt 3 -untranslated regions (UTR). The isolated cDNA detected poly(A) + RNAs of 5.4 and 3.4 kb in rat liver and kidney by Northern blot hybridization. The nt sequence of the isolated...
A gene (hag) encoding the flagellin (Fla) protein was cloned from Serratia marcescens (Sm) 8000, the wild-type strain of Sr41. The hag gene codes for a 348-amino-acid (aa) protein of 36.7 kDa. The predicted aa sequence showed 79% homology compared with the Fla of Sm 274 which has been reported previously [Harshey et al., Gene 79 (1989) 1-8]. Dot-matrix analysis of the Sm 8000 Fla showed that the...
One of three gene copies encoding ammonia monooxygenase subunit A (AmoA) and flanking sequences was isolated from genomic DNA of the chemolithotrophic soil bacterium Nitrosospira sp. NpAV using oligodeoxyribonucleotide primers and the polymerase chain reaction (PCR). The gene (amoA) encodes a 274-amino-acid polypeptide which has similarity to the ammonia monooxygenase acetylene-binding protein from...
The nucleotide (nt) sequence of the recA gene of Leptospira biflexa serovar patoc strain Patoc I has been determined. The deduced amino acid (aa) sequence of the RecA protein is 387 aa long with a predicted molecular mass of 42 355 Da. The aa sequence has a high degree of identity to the aa sequences of many bacterial RecA, including Pseudomonas fluorescens, Escherichia coli and Bacillus subtilis...
We report the isolation and characterization of genomic and cDNA clones encoding zebrafish fibroblast growth factor 3 (FGF3). An initial cDNA clone was generated by PCR amplification using degenerate oligo primers corresponding to a conserved region of protein found in the mouse and human homologues. Screening a cDNA library made from 30-33-h-old zebrafish embryos with this PCR product led to the...
The complete nucleotide (nt) sequence of the gene encoding cat serum albumin has been determined. The nt and deduced amino-acid sequences were compared to those of other known mammalian serum albumins (SA)
The mRNA differential display method was used to isolate antibacterial defense genes from Trichoplusia ni. The mRNA population in last-instar T. ni larvae injected with bacteria was compared to that of untreated larvae. Using a PCR amplified probe corresponding to an induced mRNA, we were able to clone an attacin homolog from a λ cDNA library from vaccinated larvae. The corresponding protein showed...
The gene encoding mouse ribosomal protein (r-protein) S6 is 2.7 kb in length, and is composed of five exons. The intron positions of the mouse S6 (Rps6) coincide exactly to those of the homologous human S6 (RPS6), but the last intron present in the human is absent in the mouse gene. The latter displays higher G + C content than the RPS6, both in the overall sequenced region and at the 3rd codon...
A reverse transcription-polymerase chain reaction (RT-PCR) has been developed for the cloning of coat protein-encoding genes (CP) of cymbidium mosaic virus (CyMV) isolated from three different infected species of Thai orchid: Cattleya, Mokara andOncidium . The analysis of the compiled sequences of the cDNA clones shows a single open reading frame which encoded a CyMVCP gene. The gene is 669 nucleotides...
The published sequence of the insertion element ISH28 contained many small ORFs that were difficult to interpret. We resequenced the entire element and found seven nucleotide differences. The corrected sequence of ISH28 is 938 bp long, and now reveals a single open reading frame of 828 bp. The putative protein is highly similar (49% aa identity) to the predicted transposase of ISH1.
In Escherichia coli, the products of the ruvA, ruvB and ruvC genes are all involved in the processing of recombination intermediates (Holliday structures) into recombinant molecules. We cloned a 9.4-kb DNA fragment from Pseudomonas aeruginosa PAO1 in a plasmid by functional complementation of the UV sensitivity of an E. coli strain with ruvABC deleted. In P. aeruginosa, the ruv region seemed to...
A new Clostridium cellulovorans (strain ATCC 35296) endoglucanase gene engF has been isolated and sequenced. The gene contains 1671 bp and codes for a protein containing 557 amino acids and a mass of 60.1 kDa. A putative signal peptide of 29 amino acids is present and the mature protein has a mass of 57.1 kDa. EngF does not have amino acid sequence homology to previously isolated EngB and EngD,...
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