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We have constructed a new multipurpose stable shuttle vector for the fission yeast Schizosaccharomyces pombe (Sp). Plasmid pDblet was designed to provide convenient features for molecular work and to overcome the inconveniences of previously designed Sp vectors. It contains the Sp ura4 gene as selectable marker and a new highly efficient ARS (autonomously replicating sequence) element, allowing...
The construction of vectors for use in Schizosaccharomyces pombe using the his3 + gene as a selectable marker is described. In addition, we report the construction of a genomic library in a his3 + -containing shuttle vector to facilitate the cloning of genes by complementation of mutant function in strains defective for His3 activity.
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