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In order to elucidate the molecular basis of stem cell factor (SCF, or steel factor/kit ligand) expression in Sertoli cells of rat testis, 1.5 kb of the 5′ flanking region of the SCF gene was isolated and characterized. The transcriptional start point (tsp) was identified by primer extension assay and a rapid amplification of cDNA ends (RACE) assay. A TATA box was found 29 base pairs (bp) upstream...
Colligins are collagen-binding proteins localized to the endoplasmic reticulum that belong to the superfamily of serine protease inhibitors and play a role in collagen biosynthesis. Previously, we cloned the human colligin-2 gene (CBP2) and mapped it to chromosome 11q13.15. To further characterize the CBP2 gene, we have determined its genomic structure and the 5 -flanking sequence. The CBP2 gene...
The human dopamine transporter (hDAT) is a member of the subfamily of monoamine transporters which show a common topological structure and possess significant amino acid sequence homology. We isolated and characterized the hDAT gene including about 1 kb of 5 -flanking region. The hDAT gene spans over 64 kb, consisting of 15 exons separated by 14 introns. The intron-exon structure of the hDAT gene...
We have cloned and sequenced the entire genomic fragment of the porcine LCN1 gene which encodes Tear lipocalin/von Ebner's gland protein, a member of the lipocalin superfamily highly expressed in porcine lachrymal and lingual glands. The porcine LCN1 gene is approximately 4.6kb in size and contains six protein-coding exons and a 3'-nontranslated exon. The structure of this porcine gene is highly similar,...
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