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The untranslated leader region (ULR) of the Drosophila LTR retrotransposon copia is known to be critical to the element's expression in a variety of species. Two copia ULR size variants are prevalent in natural populations. The more transcriptionally active full length variants contain within their ULRs two tandemly repeated copies of a 28-bp region of dyad symmetry with a sequence similarity to the...
The gene lac-1, encoding the enzyme laccase, is the best characterized of a number of genes in the chestnut blight fungus, Cryphonectria parasitica, that are repressed by hypoviruses, a group of virulence-attenuating mycoviruses. lac-1 has also been shown to be transcriptionally activated by low concentrations of the translational inhibitor cycloheximide (CHX) and by the immunosuppressant cyclosporin...
The 5′ upstream region (about 3.1kb) of the cellulose synthase operon (bcs operon) has been isolated by cloning from Acetobacter xylinum strain BPR 2001. The expression level of the upstream region was determined using sucrose synthase cDNA as a reporter gene in the shuttle vector pSA19. The expression occurred with the 1.1-kb upstream sequence from the ATG start codon of the bcs operon but not with...
In the present study we have analyzed the integrity and expression of the αA-crystallin gene, that codes for a major structural component of the lens, in a blind cave form of the teleostean fish, Astyanax fasciatus. This is the first αA-crystallin gene cloned from a teleostean fish. Sequence comparison of this cave-form gene with its epigean conspecific and with homologs of distantly related taxa...
Abnormal regulation of transcription of the β-amyloid precursor protein (βAPP) gene is implicated in the pathogenesis of Alzheimer's disease (AD). We have examined a 17-kb genomic DNA region which contains the 5'-flanking region (promoter), first exon and intron of the βAPP gene of the Rhesus monkey (rhβAPP). A predominant transcription start site was identified 146bp upstream of the translation initiation...
The 5' flanking region of the human β-casein gene was investigated for the presence of regulatory sequences mediating the action of the lactogenic hormones prolactin and dexamethasone. DNA encompassing 9389 base pairs of the flanking region was isolated and a sequence comparison performed with regulatory regions previously identified in the β-casein gene of rodents and ruminants. The analysis revealed...
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