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Bursaphelenchus xylophilus and Bursaphelenchus mucronatus are migratory endoparasitic nematodes that live in pine trees. To gain insight into their molecular similarities and differences, transcriptomes of the two nematodes were analysed. A total of 23,765 and 21,782 contigs (>300bp) were obtained from B. xylophilus and B. mucronatus, respectively. More than 80% of the contigs could map to each...
We determined the complete mitochondrial genome sequences for Bursaphelenchus mucronatus, one species of pinewood nematode. The genome is a circular-DNA molecule of 14,583bp (195bp smaller than its congener Bursaphelenchus xylophilus) and contains 12 protein-coding genes (lacking atp8), 22 tRNA genes, and 2 rRNA genes encoded in the same direction, consistent with most other nematodes. Based on sequence...
Bursaphelenchus xylophilus is known as the causative agent of pine wilt disease with complex life cycles. In this research, four small RNA libraries derived from different infection stages of pine wilt disease were constructed and sequenced. Consequently, we obtained hundreds of evolutionarily conserved miRNAs and novel miRNA candidates. The analysis of miRNA expression patterns showed that most miRNAs...
Temperatures directly influence the distribution and intensity of pine wilt disease, which is caused by the pine wood nematode Bursaphelenchus xylophilus. Small heat shock proteins (sHSPs) are molecular chaperones that contribute to nematode survival during the stress response to high temperatures. Seven B. xylophilus sHSPs (Bx-sHSPs) were identified and studied in a whole-genome shotgun project....
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