The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
Thiolase (Thl) is an important enzyme at the junction in the pathway leading to the production of either acids (acetate or butyrate) or solvents (acetone, butanol or ethanol) during the growth of Clostridium acetobutylicum ATCC 824. Cloning and expression of the Thl-encoding gene (thl) has been described [Petersen and Bennett, Appl. Environ. Microbiol. 57 (1991) 2735-2741], as has the purification...
Using the polymerase chain reaction (PCR) and primers based on regions of homology between the human and murine interleukin 7 (IL-7)-encoding cDNAs, we have amplified an ovine (ov) IL-7 cDNA from reverse-transcribed RNA extracted from concanavilin A (Con A)-activated ovine lymph-node cells. The nucleotide sequence of the cDNA and the predicted amino acid (aa) sequence showed significant homology to...
We have purified two distinct DNA methyltransferases from pea shoot tips and analysed their sequence specificity using synthetic oligodeoxyribonucleotide substrates and chemical sequencing methods. One methylates only CG target sequences, whereas the other methylates only CAG or CTG target sequences. We have found no evidence for methylation of the 5 cytosine in CCG target sequences either in...
Expression of the interleukin 10-encoding (IL-10) mRNA by ovine (ov-) cells, in response to mitogenic stimulation, was assessed by Northern blot and polymerase chain reaction (PCR) analyses using a human (hu) IL-10 cDNA probe and oligodeoxyribonucleotide primers based on homologous regions of the human and murine IL-10 cDNA sequences. A 315-bp cDNA generated by the PCR analysis was cloned and used...
The aspartyl-tRNA synthetase (AspRS)-encoding gene from the archaeon, Pyrococcus sp. KOD1 (KOD1), was cloned and sequenced, and expressed in Echerichia coli. The purified AspRS possessed an aminoacyation activity for tRNA extracted from KOD1. Analysis of the deduced amino-acid sequence (438 aa, 50 893 Da) revealed that the AspRS of KOD1 is a chimerical protein of bacteria and eukarya. Regional...
Typical programmed cell death requires de novo macromolecular synthesis and shares common morphological changes referred to as apoptosis. To elucidate the molecular mechanism of apoptosis, we isolated cDNA clones that are induced in various types of apoptosis by the differential display method. Among such clones, the MA-3 mRNA was induced in all apoptosis-inducible cell lines tested so far, including...
A new Clostridium cellulovorans (strain ATCC 35296) endoglucanase gene engF has been isolated and sequenced. The gene contains 1671 bp and codes for a protein containing 557 amino acids and a mass of 60.1 kDa. A putative signal peptide of 29 amino acids is present and the mature protein has a mass of 57.1 kDa. EngF does not have amino acid sequence homology to previously isolated EngB and EngD,...
The mouse His-1 gene encodes a spliced and polyadenylated RNA with no long open reading frame (ORF), making it difficult to distinguish a functional protein coding domain. To identify candidate protein coding ORFs, and other functionally significant regions, we have isolated and sequenced 8.5 kb of a human genomic DNA that is homologous to the mouse His-1 gene. Alignment of the mouse and human sequences...
A rat cDNA clone encoding the mismatch repair protein MSH2 has been isolated and characterized. The cDNA has an open reading frame of 2802 nucleotides in length coding for a protein of 933 amino acids (100 kDa). It shows significant homology to human and mouse MSH2. Northern blot analysis of rat MSH2 in the testes of rats of different ages showed maximum expression at 20 days, at which time the...
A second flavodoxin gene, distinct from the nifF gene encoding nitrogenase flavodoxin, has been isolated from Klebsiella pneumoniae. This flavodoxin gene is a homologue of the E. coli fldA gene and is located 286 bp upstream of the K. pneumoniae fur gene. Primer extension analysis revealed an unusual promoter region upstream of the K. pneumoniae fldA gene that does not match the -35/-10 consensus...
In order to investigate the role of bacterioferritin (Bfr) in the biomineralization of magnetite by microorganisms, we have cloned and sequenced the bfr genes from M. magnetotacticum. The organism has two bfr genes that overlap by one nucleotide. Both encode putative protein products of 18kDa, the expected size for Bfr subunits, and show a strong similarity to other Bfr subunit proteins. By scanning...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.