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An individual strain of Neisseria gonorrhoeae may produce up to 16 different DNA methyltransferases (MTases). We have used a novel cloning system that is able to detect MTase clones in the absence of direct selection [Piekarowicz et al., Nucleic Acids Res. 19 (1991)1831-1835] to identify 14 different MTase clones. Initial characterization of these clones indicates that at least seven of these MTases...
Overproduction of the NlaX DNA methyltransferase (M NlaX) in an Escherichia coli host conferred resistance toSso II restriction endonuclease (R SsoII) digestion. This suggested an overlap of sequence specificity between M NlaX and M SsoII, the latter of which modifies the internal cytosine of the target sequence 5 -CCNGG-3 . A variant of M NlaX (M SsoNla), containing an N-terminal extension...
The salIR and salIM genes of Streptomyces albus G encode the restriction endonuclease (ENase) and DNA methyltransferase (MTase) of the SalI restriction-modification (R-M) system. In S. albus G, the genes constitute an operon that is mainly transcribed from a promoter located upstream from salIR, the first gene of the operon. In addition, a second promoter, at the 3 end of salIR, allows independent...
We have cloned and sequenced the accIRM genes from Weeksella zoohelcum (the original identification of this strain as Acinetobacter calcoaceticus was incorrect). Our sequence differs in the coding regions from a previously published sequence by the addition of three nucleotides near the 3 end of the DNA methyltransferase-encoding gene (accIM). We have sequenced approx. 3 kb beyond this operon...
To identify the gene coding for the endonuclease which processes the 3 end of mitochondrial (mt) tRNA transcripts in Saccharomyces cerevisiae, nuclear mutations able to complement a mt mutant (Ts932) defective for this process were isolated and analyzed. One of these mutants exhibited a growth defect both on respiratory and fermentable media. Complementation of this phenotype with a S. cerevisiae...
The xmnIRM genes from Xanthomonas manihotis 7AS1 have been cloned and expressed in Escherichia coli. The nucleotide (nt) sequences of both genes were determined. The XmnI methyltransferase (MTase)-encoding gene is 1861 bp in length and codes for 620 amino acids (aa) (68 660 Da). The restriction endonuclease (ENase)-encoding gene is 959 bp long and therefore codes for a 319-aa protein (35 275 Da)...
The Saccharomyces cerevisiae APN1 gene, encoding the bifunctional DNA repair enzyme apurinic/apyrimidinic (AP) endonuclease/3 -repair diesterase, was used as a probe to isolate a gene homolog, CeAPN1, from a Caenorhabditis elegans cDNA library. The CeAPN1 gene is predicted to encode a protein 30 kDa in size, which shares 40.4% and 44.9% identity at the amino acid level with, respectively, S. cerevisiae...
The genes encoding the AatII restriction endonuclease and methylase from Acetobacter aceti have been cloned and expressed in Escherichia coli. The nucleotide sequences of aatIIM and aatIIR genes were determined. The aatIIM andaatIIR genes are 996 bp and 1038 bp, respectively, encoding the 331-aa methylase with a predicted molecular mass of 36.9 kDa, and the 345-aaAat II restriction endonuclease...
BglII, a type II restriction-modification (R-M) system from Bacillus globigii, recognizes the sequence 5 -AGATCT-3 . The system has been cloned into E. coli in multiple steps: first the methyltransferase (MTase) gene, bglIIM, was cloned from B. globigii RUB561, a variant containing an inactivated endonuclease (ENase) gene (bglIIR). Next the ENase protein (R. BglII) was purified to homogeneity...
Chicken repeat 1 (CR1) elements comprise a family of non-long terminal repeat (LTR) retrotransposons that have several noteworthy features. For example, whereas most other non-LTR elements have poly(A) tracts or other simple A-rich repeats at their 3 ends, the 3 ends of CR1 elements conform to the consensus [(CATTCTRT)(GATTCTRT) 1-3 ]. CR1 elements also display an unusual bias...
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