Platinum nanowires (PtNWs) prepared by electrodeposition method with the help of porous anodic aluminum oxide (AAO) templates have been solubilized in chitosan (CHIT) together with carbon nantubes (CNTs) to form a PtNW–CNT–CHIT organic–inorganic system. The resulting PtNW–CNT–CHIT material brings capabilities for utilizing synergic action of PtNWs and CNTs to facilitate electron-transfer process in electrochemical sensor design. The PtNW–CNT–CHIT film modified electrode offered a significant decrease in the overvoltage for the hydrogen peroxide and showed to be excellent amperometric sensors for hydrogen peroxide at −0.1V over a wide range of concentrations, and the sensitivity is 260μAmM −1 cm −2 . As an application example, by linking glucose oxidase (GOx), an amplified biosensor toward glucose was prepared. The glucose biosensor exhibits a selective determination of glucose at −0.1V with a linear response range of 5×10 −6 to 1.5×10 −2 M with a correlation coefficient of 0.997, and response time <10s. The high sensitivity of the glucose biosensor is up to 30μAmM −1 cm −2 and the detection limit was 3μM. The biosensor displays rapid response and expanded linear response range, and excellent repeatability and stability.