The controlled effects of age and weaning on the numbers of CD2 + T cells, subsets (CD4 + , CD8 + ), accessory cells (macrophage/granulocyte) and cells expressing MHC class II (DQw) and IL-2R in the piglet intestine was investigated.At birth low numbers of CD2 + CD4 - CD8 - cells were the only demonstrable T cells in the intestine. Monocyte/granulocyte and MHC class II + cells were also detected in low numbers and IL-2R + cells were proportionally quite numerous. All those cell populations, except the IL-2R + cells, increased thereafter and peaked at Week 7 when the numbers of cells were comparable with those of adult animals. CD4 + cells increased dramatically after Week 1. In contrast, CD8 + remained scarce until after 5-7 weeks of age in unweaned animals. Four days after weaning at 3 weeks old, there were increases in CD2 + (P < 0.001) and macrophage/granulocyte (P < 0.01) cells in proximal small intestinal villi and in CD2 + cells only (P < 0.01) in crypts. No significant changes in cell numbers were demonstrated in the distal small intestine.