Human longevity is a multifactorial condition with both genetic and environmental contribution. There exist a whole variety of hypotheses which try to elucidate the mechanisms of aging. One of them, free radical theory of aging, supports a role for oxidative stress as a determinant of the rate of aging. The aim of this work has been to evaluate some oxidative stress markers in Cuban centenarians. In the present study we include 350 centenarians from four different provinces of Cuba. Plasma levels of MDA, AOPPs and free thiol groups as well as the activities of SOD1 and CAT were measured. All techniques were performed using spectrophotometric methods. Levels of plasma MDA and AOPPs were highest in centenarians compared to the respective younger groups. Additionally SOD1 activity differs between the groups, showing significantly decreased in individuals between 61 to 99 years. However, this result did not apply to the centenarians group. Interestingly, CAT activity did not significantly differ between these older groups. Plasma free thiols in centenarian were significantly lower than all age group. In the present study, we report the age-dependent alterations in biomarkers of oxidative damage and confirming the occurrence of oxidative damage to biomolecules during the aging process.