This study examined pilot-scale extraction and lab-scale deacidification of rice bran oil by using supercritical carbon dioxide (SC-CO 2 ). Two purest gamma-oryzanols (γ-oryzanols) (>98wt%) were initially obtained by preparative reverse-phase high-performance liquid chromatography. Supercritical carbon dioxide extraction at 300bar and 313K from 1.03kg powdered rice bran indicated a total yield of oil of 15.7% with a free fatty acids content of 3.75%, obtained from 20.5kg of carbon dioxide in 8h. In the SC-CO 2 deacidification, pressure ranged from 200bar to 300bar, temperature ranged from 343K to 363K and consumption of carbon dioxide ranged from 900g to 2700g: the efficiency of removal of free fatty acids from 13g extracted oil in deacidification at 250bar and 353K reached 97.8% using 2700g of carbon dioxide. Finally, three-factor center composite scheme of response surface methodology was employed in designing a SC-CO 2 deacidification system, which demonstrated that the pressure and consumption of carbon dioxide are significant in retaining triglycerides and in removing free fatty acids from rice bran oil.