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To develop a rapid and reliable method for molecular diagnosis of Y-chromosomal microdeletions.Study of diagnostic accuracy.Molecular diagnostics laboratories in three hospitals.A total of 701 men with nonobstructive azoospermia or oligozoospermia from three hospitals.We developed a quadruplex real-time polymerase chain reaction (PCR) assay and evaluated its performance in molecular diagnosis of Y-chromosomal...
To characterize the deletion patterns and its breakpoints in oligozoospermic patients presenting AZFb and AZFc microdeletions and to understand the recombination mechanisms underlying these microdeletions.Case report.Genetics Department of Faculty of Medicine of Porto, Porto, Portugal.Two men with severe oligozoospermia and two men with nonobstructive azoospermia identified as having different AZFb+c...
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