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To study the effect of age and sample state on cryopreservation of testicular tissue, evaluate toxicity of commonly used cryoprotectants (CPs), and determine their optimal concentration for use.Prospective experimental study.Academic research unit.Patients with prostate carcinoma undergoing orchidectomy. We also studied immature and adult male Holtzman rats.Toxicity of CPs before freezing, morphology,...
To develop and evaluate a method to detect acrosome reaction (AR) in live human sperm.Prospective study.Basic research laboratory.Human semen samples with normal parameters obtained from healthy donors.Acrosome reaction assays.Fluorescence assessment of AR.Evaluating acrosomal exocytosis in live human sperm is challenging. In this study, we report that in reacting sperm, Pisum sativum agglutinin conjugated...
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