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Functional mouse prostaglandin E 2 (PGE 2 ) receptor EP 3α subtype has been expressed in insect cells using the baculovirus system (C. Huang and H. H. Tai, 1995,Biochem. J.307, 493-498). Site-directed mutagenesis was carried out at Thr-221, Ser-268, and Ser-272. These hydroxy amino acid residues are highly conserved among prostaglandin receptors of many species and could...
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