Three intact and four degraded hyaluronans were investigated by using chemiluminometry, differential scanning calorimetry, and thermogravimetry. Degradation of hyaluronan was induced by a system containing H 2 O 2 alone (882mM); 55mM H 2 O 2 plus 1.25μM CuCl 2 ; NaOCl alone (10mM); and NaOCl plus CuCl 2 and ascorbic acid (10mM, 0.1μM, and 100μM, respectively). The four different oxidative systems yielded biopolymer fragments represented by similar viscosity characteristics. The results obtained by using chemiluminescence and thermoanalytical methods indicate that hyaluronans of similar rheological properties could be distinguished from each other.