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The highly conserved residue F208 in protein R2 ofE. coliribonucleotide reductase is close to the binuclear iron center, and found to be involved in stabilizing the tyrosyl radical Y122• in wild type R2. Upon the reconstitution reaction of the mutant R2 F208Y with ferrous iron and molecular oxygen, we observed a new EPR singlet signal (g=2.003) formed concomitantly with decay of the transient tyrosyl...
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