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Enzymatic methylation of endogenous proteins in clonal pancreatic β-cell, HIT-T15, was investigated. When cell extract incubated with S-adenosyl-l-[methyl- 3 H]methionine was subjected to SDS-PAGE followed by fluorography, endogenous 20-kDa protein was highly [methyl- 3 H]-labeled. The increase of methylation was correlated with insulin secretion, when the cells were treated with secretagogue;...
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