Biochemical and Biophysical Research Communications

Items from 1 to 20 out of 39 results

article

Characterization of the InsP6-dependent interaction between CK2 and Nopp140

Won-Kyu Lee, Sang-Yeop Lee, Woo-Il Kim, Yoon-Hwa Rho, more

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 439-444

Nopp140, a highly phosphorylated nucleolar protein, negatively regulates CK2, a kinase essential for cell proliferation. We quantitatively analyzed the interaction between two subunits of CK2 and Nopp140 and characterized the mechanism by which InsP ₆ inhibits the interaction. Nopp140 specifically binds to the catalytic subunit of CK2 (CK2α) with a dissociation constant of (K _d ) of...

article

Tubulin assembly is disordered in a hypogeomagnetic field

Dong Liang Wang, Xing Sheng Wang, Rong Xiao, Ying Liu, more

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 363-368

Although the effect of a magnetic field on functions of many proteins has been reported, tubulin assembly in a hypogeomagnetic field (HGMF) has not yet been characterized. Here, we show disorder in tubulin self-assembly in an HGMF. Absorbance at 350nm, commonly used to monitor tubulin self-assembly, was altered in the HGMF, providing evidence for the effects of HGMF on tubulin. Measurements of intrinsic...

article

Striatal 19S Rpt6 deficit is related to α-synuclein accumulation in MPTP-treated mice

Bin Liu, Qiaoqiao Shi, Shiping Ma, Nan Feng, more

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 277-282

Striatal mitochondrial proteins were investigated using proteomics in the 1-methyl 4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson’s disease. Four proteins, 19S proteasome ATPase Rpt6 (19S Rpt6), Lectin-related nature killer cell receptor LY 49S, Zinc finger A20 domain containing 1, and the sodium channel-associated protein 1 isoform 2, were significantly decreased while α-synuclein...

article

Expression of death receptor 4 induces caspase-independent cell death in MMS-treated yeast

Mi-Sun Kang, Sung-Keun Lee, Chang-Shin Park, Ju-Hee Kang, more

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 305-309

DR4, a tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor, is a key element in the extrinsic pathway of TRAIL/TRAIL receptor-related apoptosis that exerts a preferential toxic effect against tumor cells. However, TRAIL and DR4 are expressed in various normal cells, and recent studies indicate that DR4 has a number of non-apoptotic functions. In this study, we evaluated the effects...

article

SIRTUIN 1: Regulating the regulator

Barbara Zschoernig, Ulrich Mahlknecht

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 251-255

Earlier analyses on the sirtuin family of histone deacetylases and its well-known member SIRT1 had their primary focus mostly on the identification of cellular targets exploring molecular mechanisms and functional networks in the control of metabolic homeostasis, differentiation, apoptosis and cell survival. However, only little is known about the regulation of SIRT1 itself, so far. Presently, SIRT1...

article

Variation of the oxidation state of verdoheme in the heme oxygenase reaction

Tomohiko Gohya, Michihiko Sato, Xuhong Zhang, Catharina T. Migita

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 293-298

Heme oxygenase (HO) converts hemin to biliverdin, CO, and iron applying molecular oxygen and electrons. During successive HO reactions, two intermediates, α-hydroxyhemin and verdoheme, have been generated. Here, oxidation state of the verdoheme-HO complexes is controversial. To clarify this, the heme conversion by soybean and rat HO isoform-1 (GmHO-1 and rHO-1, respectively) was compared both under...

article

Development of high-throughput screening system for osteogenic drugs using a cell-based sensor

Hironori Hojo, Kazuyo Igawa, Shinsuke Ohba, Fumiko Yano, more

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 375-379

To effectively treat osteoporosis and other bone-loss disorders, small compounds that potently induce bone formation are needed. The present study initially attempted to establish a monitoring system that could detect osteogenic differentiation easily, precisely, and noninvasively. For this purpose, we established pre-osteoblastic MC3T3E1 cells stably transfected with the GFP reporter gene driven...

article

p53 Target DDA3 binds ASPP2 and inhibits its stimulation on p53-mediated BAX activation

Wei-Tzu Sun, Pei-Chen Hsieh, Ming-Lun Chiang, Mei-Chih Wang, more

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 395-398

The p53 tumor suppressor functions in maintaining the integrity of the genome. We have previously reported that DDA3 is an oncoprotein transcriptionally regulated by p53. To explore mechanisms underlying DDA3 action, we searched for its interacting proteins by yeast two-hybrid screening, and identified ASPP2, a p53 binding protein, as its binding partner. The DDA3/ASPP2 binding was confirmed in vitro...

article

Identification of the RelA domain responsible for action of a new NF-κB inhibitor DHMEQ

Mariko Watanabe, Makoto Nakashima, Tomiteru Togano, Masaaki Higashihara, more

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 310-314

A new NF-κB inhibitor dehydroxymethylepoxyquinomicin (DHMEQ) has a potential to be applied to clinical medicine as an anti-cancer and anti-inflammatory agent. DHMEQ inhibits localization of NF-κB in the nucleus and the inhibitory effect by DHMEQ is more potent on p50/RelA than on p50 homodimer. However, a molecular target of DHMEQ is unknown. In this study, we identified residues CEGRSAGSI, which...

article

The adaptor protein Grb2 regulates cell surface Fas ligand in Schwann cells

Peter B. Thornhill, Jason B. Cohn, William L. Stanford, Julie Desbarats

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 341-346

Fas Ligand (FasL, CD178) is a cytokine that may be secreted or expressed as a transmembrane ligand at the cell surface, and induces apoptosis by binding to the “death receptor” Fas (CD95). Here, we show that Grb2, an SH3 domain-containing adaptor protein, binds to the proline-rich domain of FasL and regulates its cell surface expression. We found that knocking down Grb2 expression decreased the amount...

article

Deficient repair of 8-hydroxyguanine in the BxPC-3 pancreatic cancer cell line

Simon G. Nyaga, Althaf Lohani, Michele K. Evans

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 336-340

Elevated levels of oxidatively induced DNA lesions have been reported in malignant pancreatic tissues relative to normal pancreatic tissues. However, the ability of the pancreatic cancer cells to remove these lesions has not previously been addressed. This study analyzed the effectiveness of the pancreatic cancer cell line, BxPC-3 to repair 8-hydroxyguanine (8-OH-Gua) relative to a nonmalignant cell...

article

Icariin stimulates angiogenesis by activating the MEK/ERK- and PI3K/Akt/eNOS-dependent signal pathways in human endothelial cells

Byung-Hee Chung, Jong-Dai Kim, Chun-Ki Kim, Jung Huan Kim, more

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 404-408

We investigated the molecular effect and signal pathway of icariin, a major flavonoid of Epimedium koreanum Nakai, on angiogenesis. Icariin stimulated in vitro endothelial cell proliferation, migration, and tubulogenesis, which are typical phenomena of angiogenesis, as well as increased in vivo angiogenesis. Icariin activated the angiogenic signal modulators, ERK, phosphatidylinositol 3-kinase (PI3K),...

article

Interaction of USF1/USF2 and α-Pal/Nrf1 to Fmr-1 promoter increases in mouse brain during aging

S. Prasad, Kanchan Singh

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 347-351

Fragile X syndrome is caused due to silencing of FMR-1 gene transcription leading to loss of fragile X mental retardation protein (FMRP). To investigate whether the transcriptional mechanism is linked to aging, we have studied interaction of the transcription factors USF1/USF2 and α-Pal/Nrf1 to E-box and GC-box, respectively, in Fmr-1 promoter in the brain of young, adult, and old mouse using electrophoretic...

article

Roles of mono-ubiquitinated Smad4 in the formation of Smad transcriptional complexes

Bei Wang, Hiroyuki Suzuki, Mitsuyasu Kato

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 288-292

TGF-β activates receptor-regulated Smad (R-Smad) through phosphorylation by type I receptors. Activated R-Smad binds to Smad4 and the complex translocates into the nucleus and stimulates the transcription of target genes through association with co-activators including p300. It is not clear, however, how activated Smad complexes are removed from target genes. In this study, we show that TGF-β enhances...

article

Antiviral potency of a siRNA targeting a conserved region of coxsackievirus A24

Eun Jung Jun, Young Ran Nam, Jeonghyun Ahn, Hungwon Tchah, more

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 389-394

Coxsackievirus A24 (CVA24) is responsible for acute hemorrhagic conjunctivitis, a highly contagious eye disease for which no prevention or treatment is currently available. We thus assessed the antiviral potential of a small interfering RNA (siRNA) targeting CVA24. HeLa cells with or without four different siRNAs complementary to 2C or 3D genome region, were challenged with various CVA24s. Among several...

article

Regulation of hepatic PPARγ2 and lipogenic gene expression by melanocortin

Nicole J. Poritsanos, Davie Wong, Maria E. Vrontakis, Tooru M. Mizuno

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 384-388

The central melanocortin system regulates hepatic lipid metabolism. Hepatic lipogenic gene expression is regulated by transcription factors including sterol regulatory element-binding protein 1c (SREBP-1c), carbohydrate responsive element-binding protein (ChREBP), and peroxisome proliferator-activated receptor γ2 (PPARγ2). However, it is unclear if central melanocortin signaling regulates hepatic...

article

Editorial Board

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > IFC

article

Characterisation of the Plasmodium vivax Pv38 antigen

Alvaro Mongui, Diana I. Angel, Catalina Guzman, Magnolia Vanegas, more

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 326-330

This study describes the identification and characterisation of Pv38, based on the available genomic sequence of Plasmodium vivax and previous studies done with its Plasmodium falciparum homologue: Pf38. Pv38 is a 355 amino acid long peptide encoded by a single exon gene, for which orthologous genes have been identified in other Plasmodium species by bioinformatic approaches. As for Pf38, Pv38 was...

article

NF-κB p50 promotes tumor cell invasion through negative regulation of invasion suppressor gene CRMP-1 in human lung adenocarcinoma cells

Ming Gao, Pei Yen Yeh, Yen-Shen Lu, Wen Ching Chang, more

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 283-287

Lung adenocarcinoma Cl1-5 cells were selected from parental Cl1-0 cells based on their high metastatic potential. In a previous study, CRMP-1, an invasion suppressor gene, was shown to be suppressed in Cl1-5 cells. However, the regulation of CRMP-1 expression has not been explored. In this study, we showed nuclear factor-κB controls CRMP-1 expression. The electromobility shift assay showed that while...

article

Extracellular matrix interacts with interferon α protein: Retention and display of cytotoxicity

Kimiko Yoshida, Atsushi Kondoh, Kenta Narumi, Teruhiko Yoshida, more

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 299-304

We have been investigating the efficacy of an intratumoral interferon (IFN)-α gene transfer against solid cancers, and found that when the gene is transduced into the subcutaneous tumors, IFN-α concentration is markedly increased in the injected tumor but not in the serum. To explain this effective confinement of IFN-α to target tissues, we hypothesized that the extracellular matrix in the tumors...

Number:
2
Volume:
376
Year:
2008

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Keywords

APOPTOSIS (3)
DIFFERENTIATION (2)
DNA REPAIR (2)
19S ATPASE RPT6 (1)
53BP2 (1)
8-HYDROXYGUANINE (1)
ACUTE HEMORRHAGIC CONJUNCTIVITIS (1)
ADAPTIN Β (1)
ADENYLATE CYCLASE (1)
AGING (1)
AMORPHOUS OLIGOMER (1)
AMP-ACTIVATED PROTEIN KINASE (AMPK) (1)
ANGIOGENESIS (1)
ANTAGONIST (1)
ANTIVIRAL EFFECT (1)
ARCHAEA (1)
ASPP2 (1)
ASTROCYTES (1)
ATB0,+ (1)
AXIAL COORDINATION STRUCTURE (1)
BACTERIAL DNA (1)
BASE EXCISION REPAIR (1)
BAX (1)
BIODEGRADATION (1)
BLOOD–BRAIN BARRIER (1)
BRAIN (1)
C-SKI (1)
CAMP IMAGING (1)
CANCER (1)
CARNITINE (1)
CASPASE-INDEPENDENT (1)
CAVEOLAE (1)
CDNA MICROARRAY (1)
CELL ENGRAFTMENT (1)
CELL SURFACE MARKER (1)
CELL THERAPY (1)
CELL-WALL-ASSOCIATED PROTEIN (1)
CELLULAR STRESS (1)
CHLOROAROMATICS (1)
CHOLERA TOXIN (1)
CIS-ACTING REPLICATION ELEMENT (1)
CK2 (1)
COL1A1GFP-MC3T3E1 (1)
COLLAGEN-INDUCED ARTHRITIS (1)
COXSACKIEVIRUS A24 (1)
CPG MOTIFS (1)
CRMP-1 (1)
CYTOKINES (1)
DDA3 (1)
DHMEQ (1)
DIENELACTONE HYDROLASE (1)
DILATED CARDIOMYOPATHY (1)
DNA DAMAGE (1)
DNA LIGASE (1)
DNA MICROARRAY (1)
DR4 (1)
DRMS (1)
EBH (1)
EDEMA TOXIN (1)
ELECTRIC FIELD (1)
ELECTRON MICROSCOPY (1)
ELECTROPERMEABILIZATION (1)
ELECTROPORATION (1)
EMSA (1)
EPI-NCSC (1)
EPR (1)
EVOLUTION (1)
EXTRACELLULAR MATRIX (1)
FAS (CD95) (1)
FAS LIGAND (CD178) (1)
FAT (1)
FATTY LIVER (1)
FLEXIBILITY (1)
FMR-1 (1)
FUNCTIONAL DOMAIN (1)
FUSION APPROACH (1)
GENE EXPRESSION (1)
GENE TRANSFER (1)
GRB2 (1)
HEART FAILURE (1)
HEME DEGRADATION (1)
HEME OXYGENASE (1)
HEMOGLOBIN FUSION (1)
HEPATIC FIBROSIS (1)
HEPATIC STELLATE CELL (1)
HIF ANTISENSE TRANSCRIPT (1)
HIF PROLYL HYDROXYLASE (1)
HISTIDINE KINASE (1)
HISTONE DEACETYLASES (1)
HJC (1)
HJM (1)
HOGG1 (1)
HUMAN BRAIN MICROVASCULAR ENDOTHELIAL CELLS (1)
HUMAN STUDY (1)
HUMAN UMBILICAL VEIN ENDOTHELIAL CELL (1)
HYPERTROPHY (1)
HYPOGEOMAGNETIC FIELD (1)
HYPOGLYCEMIA (1)
HYPOXIA-INDUCIBLE FACTOR (1)
HYPOXIA-INDUCIBLE FACTOR 1Α SUBUNIT INHIBITOR (1)
more

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Biochemical and Biophysical Research Communications

Characterization of the InsP6-dependent interaction between CK2 and Nopp140

Tubulin assembly is disordered in a hypogeomagnetic field

Striatal 19S Rpt6 deficit is related to α-synuclein accumulation in MPTP-treated mice

Expression of death receptor 4 induces caspase-independent cell death in MMS-treated yeast

SIRTUIN 1: Regulating the regulator

Variation of the oxidation state of verdoheme in the heme oxygenase reaction

Development of high-throughput screening system for osteogenic drugs using a cell-based sensor

p53 Target DDA3 binds ASPP2 and inhibits its stimulation on p53-mediated BAX activation

Identification of the RelA domain responsible for action of a new NF-κB inhibitor DHMEQ

The adaptor protein Grb2 regulates cell surface Fas ligand in Schwann cells

Deficient repair of 8-hydroxyguanine in the BxPC-3 pancreatic cancer cell line

Icariin stimulates angiogenesis by activating the MEK/ERK- and PI3K/Akt/eNOS-dependent signal pathways in human endothelial cells

Interaction of USF1/USF2 and α-Pal/Nrf1 to Fmr-1 promoter increases in mouse brain during aging

Roles of mono-ubiquitinated Smad4 in the formation of Smad transcriptional complexes

Antiviral potency of a siRNA targeting a conserved region of coxsackievirus A24

Regulation of hepatic PPARγ2 and lipogenic gene expression by melanocortin

Editorial Board

Characterisation of the Plasmodium vivax Pv38 antigen

NF-κB p50 promotes tumor cell invasion through negative regulation of invasion suppressor gene CRMP-1 in human lung adenocarcinoma cells

Extracellular matrix interacts with interferon α protein: Retention and display of cytotoxicity

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