A lanthanide-based luminescent probe [EuL] for Zn 2+ was synthesized utilizing a DO3A ring as the Eu 3+ chelator and a DPA moiety as the Zn 2+ chelator. A quinoxaline functioned as both the linker and antenna. The two nitrogen atoms in quinoxaline are involved in the Eu 3+ and Zn 2+ binding, respectively. It gave an excitation at 336nm for [EuL], which was longer than the reported values of this type of probes at present. Moreover, the titration studies provided a dissociation constant (K d ) of 55nM for [EuL] while the competition investigation indicated that [EuL] had an affinity comparable to that of TPA (K d 0.014nM).