Anthocyanins, a group of polyphenolic pigments, have been proved to possess various bioactivities. However, they are unstable in the small intestine and absorbed with low bioavailability. The discrepancy between the low bioavailability of anthocyanins and their good bioactivities has not been illuminated yet. Moreover, information about the digested property and stability of purple rice anthocyanins in the alimentary tract is still limited. Thus, the present work was designed to study the digestion property and the changes in antioxidant and cytoprotective activities of purple rice anthocyanins using an in vitro digestion model, and to investigate the interactions between gastric and intestinal digested anthocyanins. The results showed that anthocyanins amount and antioxidant and cytoprotective effects didn't change significantly during gastric digestion. However, about 76% of total anthocyanins were degraded during intestinal digestion. The IC50 values of intestinal digested sample tested by DPPH and ABTS methods were about 19.1 and 16.9μg/mL, respectively, far higher than that of non-digested sample (about 7.7 and 7.1μg/mL, respectively). Cytoprotective effect of intestinal digested sample also decreased significantly. Synergistic effects on antioxidant and cytoprotective activities were observed between the gastric and intestinal digested samples at a relative low concentration. Those results suggest that the bioactivities of purple rice anthocyanins may be changed after digestion and enhanced through the synergies between their gastric and intestinal digested catabolites.