Indirect, non-competitive amperometric immunoassay for accurate quantification of calpastatin, a meat tenderness marker, in bovine muscle

Kinga Zór; Katarzyna Dymek; Roberto Ortiz; Audrey M. Faure; Ebru Saatci; Lo Gorton; Ronald Bardsley; Mihaela Nistor

doi:10.1016/j.foodchem.2012.01.065

Analytical Methods
Indirect, non-competitive amperometric immunoassay for accurate quantification of calpastatin, a meat tenderness marker, in bovine muscle

Kinga Zór, Katarzyna Dymek, Roberto Ortiz, Audrey M. Faure, Ebru Saatci, Lo Gorton, Ronald Bardsley, Mihaela Nistor

Source

Food Chemistry > 2012 > 133 > 2 > 598-603

Abstract

A method is described for quantification of the beef tenderness marker, calpastatin, in meat samples by amperometric detection. Using a novel bovine recombinant partial calpastatin protein as standard antigen a low detection limit of 0.2ng/mL was achieved. The influence of the complex matrix was minimised by heat pretreatment and dilution of the samples prior to detection of calpastatin. The relative error between the direct sample measurement and standard addition methods was 5.89%, confirming the accuracy of the developed amperometric immunoassay.