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Enumeration and separation of the toxic dinoflagellate Alexandrium minutum from natural samples using immunological procedures with blocking antibodies
A. minutum cells from natural seawater samples were counted using an immunofluorescent procedure and flow cytometry and separated using immunobeads. The cell densities estimated by flow-cytometry showed an excellent concordance with the direct count estimations in some samples and slight underestimation in others. Flow cytometer counts by immunological procedures showed a higher repeatability than direct counts for low cell density. Immunobeads were able to successfully separate a high percentage of A. minutum cells from natural samples with relatively little mistaken separation.