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The mutagenic thymidine analog bromodeoxyuridine (BrdUrd) may incorrectly incorporate opposite deoxyguanine in DNA, then pair with deoxyadenosine during subsequent replication. It appears to preferentially target the 3 -G of 5 -NGGN-3 sequences in mammalian cells in culture to induce G → A transitions. Ras genes should therefore be vulnerable to activation by mutation at glycine codons 12 (GGT)...
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