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Flow cytometry was used to analyze the DNA content of nuclei isolated from four different shoot cultures growing in vitro, and from callus of Rubus chamaemorus. The protocol for isolation of the nuclei of this species was optimized. Flow cytometry proved to be a fast and accurate method for estimation of the genome size of R. chamaemorus, a species containing phenolic compounds in the cytosol, which...
After 12 h incubation at 35 o C, cell walls isolated from bean calluses released approximately 3% of the cell wall dry weight as pectic polysaccharides (2.7%), largely composed of a t-1,2-diaminecyclohexane-N,N,N',N'-tetraacetic acid-soluble homogalacturonan, and monosaccharides (0.3%). The release of sugars occurred over a broad pH range and was affected by the type of buffer used in cell...
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