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Ploidy stability in gynogenic clones of long-day onion is assessed during micropropagation. In order to induce chromosome doubling in haploids, explants and plantlets of three clones were treated in vitro. Colchicine (0.625-12.5 mM) and oryzalin (10-200 μM) were tested for 24 h in combination with culture conditions (4°C in the dark or 24°C in the 16 h day photoperiod). Diploidization performed...
Successful application of doubled-haploid (DH) technology in onion (Allium cepa L.) breeding programs requires production of large numbers of fertile DH plants. Only a small percentage of the onion plants recovered via gynogenesis are spontaneous DH, so induction of chromosome doubling is required. The chromosome doubling step is currently one of the major limitations in onion DH programs. To address...
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