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A rapid, signal step and sensitive immunochromatographic (IC) test for detection of aflatoxin B 1 (AFB) in foods was described. Colloidal gold-labeled polyclonal antibody specific to AFB was used as the marker; AFB–BSA conjugate was the competitive antigen and goat-anti-rabbit antibody as control. Conditions for the analysis of AFB to be completed in less than 10min were optimized. With visual...
One hundred and thirty seven samples of melon seeds (Colocynthis citrullus L.) from randomly selected farmers’ stores in the humid forest and Northern Guinea savanna of Nigeria were analysed for the incidence of diseased seeds, moisture content, associated moulds and levels of aflatoxin B 1 contamination. The proportion of diseased seeds ranged from 2.5 to 37.3% in the forest and 2.1 to 17...
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