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The α-acetolactate decarboxylase (ALDC) gene from Acetobacter aceti subsp. xylinum was integrated stably into the chromosome of a brewer's yeast. Location of the integrated ALDC gene was analyzed by Southern hybridization and CHEF (contour clamped homogeneous electric field). It was confirmed that the ALDC gene was integrated in the rRNA locus of the chromosome XII. Laboratory-scale brewing tests...
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