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Eschericha coli was genetically engineered to produce recombinant human bone morphogenetic protein-2 (rhBMP-2) in a non-active aggregated form using a temperature-inducible expression system. High concentrations of both biomass (75 g cell dry weight per liter of culture broth) and inactive rhBMP-2 (8.6 gl -1 ) were obtained by applying a high-cell-density cultivation procedure. After...
Acclimation patterns in kinetic coefficients clearly demonstrate the limits of Monod's theory for the mathematical description of microbial growth. Focusing on E. coli grown under variable glucose levels, these patterns turn out to be highly diverse and sometimes even contradictory. Here, a new model based on an optimisation assumption is applied to a spectrum of adaptation phenomena, which are observed...
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