Journal of Biotechnology

article

Evaluation of the E. coli ribosomal rrnB P1 promoter and phage-derived lysis genes for the use in a biological containment system: A concept study

Karsten Tedin, Angela Witte, Günter Reisinger, Werner Lubitz, more

Journal of Biotechnology > 1995 > 39 > 2 > 137-148

A concept study devised for the development of a biological containment system has been conducted. We show that the lysis genes of different phage origin function in a variety of bacteria. They may therefore be suited for conditional suicide cassettes. Moreover, we tested whether the Escherichia coli rrnB P1 promoter could function as an environmentally responsive element sensing poor growth conditions...

article

Concentration of BSA using a superabsorbent polymer: process evaluation

D.M.F. Prazeres

Journal of Biotechnology > 1995 > 39 > 2 > 157-164

A commercially available super absorbent polymer from Hoechst (Sanwet IM-5000-SG) was tested for the concentration of dilute solutions of bovine serum albumin (BSA). A systematic study was undertaken in order to evaluate the possibility of scaling-up the process. The polymer was first characterized by determining the swelling ratio (or mass increase) in aqueous solution as a function of time, temperature,...

article

Evaluation of Escherichia coli recBC sbcBC mutants for cloning by recombination in vivo

Eric Degryse

Journal of Biotechnology > 1995 > 39 > 2 > 181-187

In vivo recombination as a tool for plasmid construction was analyzed using a model system based on the properties of the RecF pathway in Escherichia coli. This pathway was used after a double strand break (DSB) effected by restriction enzyme cleavage of the plasmids of interest. DSB repair was shown to be independent of the methylation state of the vector or insert DNA, of the type of restriction...

article

Polymerase chain reaction and non-radioactive gene probe based identification of mosquito larvicidal strains of Bacillus sphaericus and monitoring of B. sphaericus 1593M, released in the environment

M. Shanmugavelu, V. Sritharan, Kunthala Jayaraman

Journal of Biotechnology > 1995 > 39 > 2 > 99-106

In attempts towards an accurate monitoring in the environment, the status of the deliberate release of Bacillus sphaericus, a powerful mosquito larvicidal agent, as well as to evolve a rapid method of screening for potent isolates of B. sphaericus, we have developed a polymerase chain reaction (PCR) method of analysis. Using specific primers spanning the 5 and 3 ends of the coding sequences of...

article

Large-scale preparation and biological activity of recombinant human parathyroid hormone

J. Paulsen, D. Ochs, M. Harder, C. Duvos, more

Journal of Biotechnology > 1995 > 39 > 2 > 129-136

Human parathyroid hormone (hPTH) has been bacterially expressed in bioreactors as cro-β-galactosidase-hPTH fusion protein. We have developed a large-scale purification scheme that exploits the pH-dependent differential solubility of hPTH and a two-step chromatographic procedure. We demonstrate that in a number of assay systems, the recombinant material obtained by this procedure is biologically active.

article

Surfactant-induced non-lethal release of anthraquinones from suspension cultures of Morinda citrifolia

Lucilla Bassetti, Marc Hagendoorn, Johannes Tramper

Journal of Biotechnology > 1995 > 39 > 2 > 149-155

A new approach based on the use of the surfactant Pluronic F-68 to obtain non-lethal release of plant cell intracellular products was investigated. Suspension cultures of Morinda citrifolia (Rubiaceae), producing anthraquinones as secondary metabolites, were selected as model system. By supplementing the growth medium with 2% w/v of Pluronic F-68 for four repeated batch cycles, cells released anthraquinones...

article

Comparison of the energetic efficiencies of hydrogen and oxychemicals formation in Klebsiella pneumoniae and Clostridium butyricum during anaerobic growth on glycerol

B.O. Solomon, A.-P. Zeng, H. Biebl, H. Schlieker, more

Journal of Biotechnology > 1995 > 39 > 2 > 107-117

Data for the anaerobic growth of Klebsiella pneumoniae DSM 2026 and Clostridium butyricum DSM 5431 on glycerol have been analyzed using the concept of material and available electron balances with consideration for hydrogen production. Models for the kinetics of energetic efficiencies of product formation under low residual glycerol are presented. For Klebsiella pneumoniae, the specific rates of...

article

The effect of fatty acids on hybridoma cell growth and antibody productivity in serum-free cultures

M. Butler, N. Huzel

Journal of Biotechnology > 1995 > 39 > 2 > 165-173

A murine B-lymphocyte hybridoma (CC9C10) was adapted for growth in a serum-free medium. Supplementation of the medium with cis-unsaturated fatty acids (10-50 μM) improved the cell yield in the order oleic/linoleic > linoleic > oleic. Initial supplementation with the fatty acids also caused a significant increase (58%) in the volumetric Mab titre. Continued growth of the cells in the fatty...

article

Aromatic ring oxidation of vanillyl and veratryl alcohols by Lentinus edodes: possible artifacts in the lignin peroxidase and veratryl alcohol oxidase assays

Claudia Crestini, Giovanni Giovannozzi Sermanni

Journal of Biotechnology > 1995 > 39 > 2 > 175-179

The degradation pathway of vanillyl and veratryl alcohol by Lentinus edodes extracellular enzymes was studied. In both cases several products of side chain oxidation and aromatic ring cleavage were isolated and characterized. We have observed that the products from veratryl alcohol degradation by Lentinus edodes are quite different from those isolated from incubations with other white-rot fungi which...

article

Production of selenomethionine-labeled recombinant human neutrophil collagenase in Escherichia coli

M. Walid Ooronfleh, T.F. Ho, P.G. Brake, T.M. Banks, more

Journal of Biotechnology > 1995 > 39 > 2 > 119-128

Molecular analogs of amino acids can be incorporated into proteins. The amino acid analog selenomethionine (SeMet) has been shown to be efficiently incorporated into the proteins of growing Escherichia coli. SeMet-containing proteins are known to produce sufficiently strong anomalous scatter permitting the solution of the selenomethionyl crystal structure by multiwavelength anomalous diffraction...

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Journal of Biotechnology

Evaluation of the E. coli ribosomal rrnB P1 promoter and phage-derived lysis genes for the use in a biological containment system: A concept study

Concentration of BSA using a superabsorbent polymer: process evaluation

Evaluation of Escherichia coli recBC sbcBC mutants for cloning by recombination in vivo

Polymerase chain reaction and non-radioactive gene probe based identification of mosquito larvicidal strains of Bacillus sphaericus and monitoring of B. sphaericus 1593M, released in the environment

Large-scale preparation and biological activity of recombinant human parathyroid hormone

Surfactant-induced non-lethal release of anthraquinones from suspension cultures of Morinda citrifolia

Comparison of the energetic efficiencies of hydrogen and oxychemicals formation in Klebsiella pneumoniae and Clostridium butyricum during anaerobic growth on glycerol

The effect of fatty acids on hybridoma cell growth and antibody productivity in serum-free cultures

Aromatic ring oxidation of vanillyl and veratryl alcohols by Lentinus edodes: possible artifacts in the lignin peroxidase and veratryl alcohol oxidase assays

Production of selenomethionine-labeled recombinant human neutrophil collagenase in Escherichia coli

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