Six new compounds, wikstronin A (1), wikstronin B (2), wikstresinol (3), acetylwikstresinol (4), bis-5′,5′-(+)-matairesinol (5), bis-5,5′-(+)-matairesinol (6), together with 20 known compounds (7–26) were isolated from the CH2Cl2 extract of roots of Wikstroemia indica. Structures of compounds 1–6 were determined by extensive NMR and CD spectroscopic analysis. In vitro preferential cytotoxicity of all the isolates was evaluated against a PANC-1 human pancreatic cell line. Compounds 8 and 12 displayed mild preferential cytotoxicity in the nutrient-deprived medium (NDM) and without causing toxicity in normal nutrient-rich conditions.